The purification of citrate-condensing enzyme.
نویسندگان
چکیده
The citrate-condensing enzyme was isolated as a crystalline protein from pig heart by Ochoa et al. (1). These workers reported that pig heart contained considerable quantities of the condensing enzyme. We were surprised when we obtained much higher yields of this enzyme from pig heart tissue with a different extraction procedure (2). This observation led to a purification procedure that yields 30 mg of crystalline condensing enzyme from 100 g of pig heart in 3 to 4 days. Using the new extraction procedure, we have also reinvestigated the condensing enzyme content in a number of tissues. Sedimentation and diffusion coefficients were determined for this enzyme, and its approximate molecular weight and turnover number were calculated.
منابع مشابه
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 236 شماره
صفحات -
تاریخ انتشار 1961